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| 产品名称： | PC-12 |
|---|---|
| 商品货号： | TS259070 |
| <strong>Cell line </strong>： | PC-12 |
| DSMZ no. ： | TS259070 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | adrenal pheochromocytoma |
| Origin ： | established from a transplantable rat adrenal pheochromocytoma in 1976; cells were described in the literature to synthesize catecholamines (dopamine, norepinephrine) and in response to nerve growth factor (NGF) a neuronal phenotype could be induced reversibly |
| Reference(s) ： | 14576, 14716 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small cells growing in clumps in suspension, adhering poorly to plastic; image; image; image |
| Medium ： | 85% RPMI 1640 + 10% horse serum + 5% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every 3-4 days; cells grow in suspension without collagen; using collagen (Collagen R- Roche at 30 µg/ml) cells grow adherent, faster and to a higher density; the adherent cells may be detached mechanically without trypsin, for single cells the suspension should be passed several times through a syringe with a 22-gauge needle; seed out at ca. 5-15 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | about 1-2 x 106 cells/ml or 30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat hypodiploid karyotype with 20% polyploidy - 39(38-40)<2n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |