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| 产品名称： | HELA-S3 |
|---|---|
| 商品货号： | TS259072 |
| <strong>Cell line </strong>： | HELA-S3 |
| DSMZ no. ： | TS259072 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | cervix carcinoma |
| Origin ： | cell line is a subclone of its parent HELA (DSM ACC 57) derived in 1955 |
| Reference(s) ： | 14859 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial, adherent cells growing as monolayer; image; image; image; image |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:8 every 3-4 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | harvest of about 7-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | confirmed as HELA-derivative, but otherwise unique DNA profile using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 32% polyploidy - 68(53-68)<3n>XX, -X, +1, -2, -4, +5, +8, +12, -13, -14, -20, -21, -22, -22, +4mar - del(1)(p35), der(1)t(1;3) (p11;q11), der(3)t(3;5)(q11;q11), i(5p)x2, del(6)(q23), add(6)(q27), del(7)(q32), i(8q), add(8)(q24), del(9)(p22p23), t(11;19)(q13;q13), add(12)(q23)x2, add(13)(p12), i(16q), add(19)(p13), add(21)(p11), i(22q) - high level of spontaneous aberrations - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |