微信小李
微信小章
产品简介
购买须知
| 产品名称： | BL-41 |
|---|---|
| 商品货号： | TS259074 |
| <strong>Cell line </strong>： | BL-41 |
| DSMZ no. ： | TS259074 |
| Species ： | confirmed originally as human with IEF of AST, NP and later by STR typing |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the tumor tissue of an 8-year-old Caucasian boy with Burkitt lymphoma; cells were described in the literature to be EBV-negative |
| Reference(s) ： | 14714, 14434 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells growing non-adherent in suspension; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 µM mercaptoethanol) |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml (initially in a 24-well-plate); split saturated culture 1:3 to 1:5 every 2 days; optimal density at about 1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | saturation density at about 2.0 x 106cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 7% polyploidy - 48(42-49)<2n>XY, +7, -13, +2mar, add(8)(q24), t(8;14)(q24;q32), der(15)t(13;15)(q13;p11), add(17)(p12), subclonal rearrangements at 1q23, 7p22, 11q13 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |