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| 产品名称： | KB-3-1 |
|---|---|
| 商品货号： | TS259075 |
| <strong>Cell line </strong>： | KB-3-1 |
| DSMZ no. ： | TS259075 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | the parental continuous cell line HELA was established from the primary cervix carinoma of a 31-year-old black woman 1951; KB-3-1 is a derivative of HELA (DSM ACC 57) and is used in multiple drug resistance studies giving raise to drug-resistant mutants (e.g. KB-V1 resistant to vinblastine; KB-CHR-8-5 resistant to colchicine); cells express MYC mRNA; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111 |
| Reference(s) ： | 14351, 14540 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, adherent cell line growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 1 mM sodium pyruvate |
| Subculture ： | split confluent culture up to 1:10 using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | about 6-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 1.8% polyploidy - 76(73-78)<3n>XX, -X, -1, -3, -4, +6, +7, +8, -9, -18, -20, +11mar, der(1)t(1;?)((p11;?), der(3)t(3;?)(p11;?), der(3) t(3;?)(p14;?), der(3p;5q), del(6)(q21), del(7)(q31), der(8)t(8;?11) (q24;q11), der(9) t(9;?)(q34;?), der(12)t(12;?)(p12;?), der(19)t(19;?)(q13;?) - supernumerary single dm present in 11% cells - 3 or more HELA markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |