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| 产品名称： | NCI-H929 |
|---|---|
| 商品货号： | TS259083 |
| <strong>Cell line </strong>： | NCI-H929 |
| DSMZ no. ： | TS259083 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP, PEP B and later by STR fingerprinting |
| Cell type ： | multiple myeloma |
| Origin ： | established from the pleural effusion of a 62-year-old white woman with myeloma (IgAkappa) at relapse; cells were described as follows: cells synthesize high amounts of immunoglobulin, MYC proto-oncogene is rearranged and MYC RNA is expressed, cells are EBV-negative; patented cell line |
| Reference(s) ： | 14486, 14549 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells or clustered in small clumps in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 50 µM mercaptoethanol |
| Subculture ： | cells are very difficult to culture; seed out at 1 x 106 cells/ml; cultivation in 12- or 24-well plates is highly recommended; maintain at a minimum of 1 x 106 cells/ml; after thawing cells grow very slowly and viability may be initially low (possibly no growth during the first week); arising debris may mimick a bacterial contamination |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | maximum density at 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR -, cyIgG -, cyIgM -, cykappa +, cylambda -; image |
| Fingerprint ： | unique DNA profile using multiplex PCR at D1S80, D2S44, D17S5 and ApoB |
| Cytogenetics ： | human hypodiploid karyotype with 16% polyploidy - 45(43-46)<2n>X, -X, +8, -10, -13, +mar, dup(1)(q11q25), del(1)(p11p25), del(6)(q25), der(8)t(8;?)(q23;?)x2, del(9)(p13), del(12)(p12) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |