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| 产品名称： | SIMA |
|---|---|
| 商品货号： | TS259084 |
| <strong>Cell line </strong>： | SIMA |
| DSMZ no. ： | TS259084 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | neuroblastoma |
| Origin ： | established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of European origin with neuroblastoma (stage III) in 1991 |
| Reference(s) ： | 14733 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cell shape is bipolar, cells grow as loosely-adherent monolayers or by forming foci; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA or by tapping the flask; seed out at ca. 5 x 106 cells/80 cm2; cells may grow slowly initially forming non-adherent large cell aggregates |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | highly variable about 34-100 hours |
| Harvest ： | cell harvest of about 0.3-0.6 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | unique DNA profile using multiplex PCR at D1S80, D2S44, D17S5 and ApoB |
| Cytogenetics ： | human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17) together with NMYC amplification is restricted to neuroblastoma - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |