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| 产品名称： | PA-TU-8988T |
|---|---|
| 商品货号： | TS259086 |
| <strong>Cell line </strong>： | PA-TU-8988T |
| DSMZ no. ： | TS259086 |
| Species ： | originally confirmed as human with IEF of AST, MDH and recently by STR fingerprinting |
| Cell type ： | pancreas adenocarcinoma |
| Origin ： | established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman; sister cell line of PA-TU-8988S (DSM ACC 204) |
| Reference(s) ： | 14508 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers; image; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 5% h.i. FBS + 5% horse serum + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5-10% CO2 |
| Doubling time ： | about 25-30 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as PA-TU-8988S using multiplex PCR at D1S80, D2S44, D17S5 and ApoB |
| Cytogenetics ： | human near-triploid karyotype with 2% polyploidy - 68(63-70)<3n>XXX, -4, +6, +6, +8, +11, -15, -16, -17, -19, +20, -21, -22, +mar, der(1)t(1;?)(p11;?), i(6p), del(6)(q23)x2, der(8)t(8;?)(q24.3;?), der(8)t(8;?)(p11;?)x2, der(11)t(11;?)(p13;?)x2, del(12)(p12.3), dup(14(q11q12), del(17)(p12), der(17)t(17;?)(p13;?) - sideline with additional der(3)t(3;?)(q11;?) - 1p11 and 8q24 breakpoints previously reported in pancreatic cancer |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |