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| 产品名称： | CACO-2 |
|---|---|
| 商品货号： | TS259091 |
| <strong>Cell line </strong>： | CACO-2 |
| DSMZ no. ： | TS259091 |
| Species ： | originally confirmed as human with IEF of MDH and NP, later by STR typing |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the primary colon tumor (adenocarcinoma) of a 72-year-old Caucasian man in 1974; cells are described to produce mucus. All uses of cell line CACO-2 (DSMZ TS259091), other than for research by academic entities, require a license from NaviCyte Scientific, Berkeley CA. For that please contact NaviCyte Scientific via email (contact@navicyte-scientific.com) |
| Reference(s) ： | 14368, 14538, 14903 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial adherent cells; after splitting cells start to grow in colonies; image; image; image;image |
| Medium ： | 80% MEM (with Earles salts) + 20% h.i. FBS + 1x non-essential amino acids |
| Subculture ： | cells grow slowly; seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 every 5-7 days using trypsin/EDTA or, if necessary, a cell scraper; after thawing cells might start slowly to take up growth; to avoid prolonged trypsinization time (>10 min) and decreased viability, split the culture before complete confluency |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | about 5-8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiologcal culture, RNA hybridization, PCR assay |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |