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| 产品名称： | MHH-ES-1 |
|---|---|
| 商品货号： | TS259092 |
| <strong>Cell line </strong>： | MHH-ES-1 |
| DSMZ no. ： | TS259092 |
| Species ： | confirmed originally as human with IEF of AST, MDH |
| Cell type ： | Ewings sarcoma |
| Origin ： | established from the ascites of a 12-year-old Turkish boy with Ewings sarcoma of the left pelvis (with peritoneal metastasis) |
| Reference(s) ： | 15155 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells of variable size, single or in small clusters, growing as monolayer; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1.5-2 x 106 cells/80 cm2; split confluent culture 1:3 -1:5 every 2-3 days using trypsin/EDTA; cells may grow rather fast |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca 35-40 hours |
| Harvest ： | about 10-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | unique DNA profile using multiplex PCR at D1S80, D2S44, D17S5 and ApoB |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 49(46-51)<2n>XXY, +X, +1, +8, +12, -17, dup(1)(q31.1q32.2), del(3)(p11), der(4)t(4;?6)(p14;p11), t(6;7)(q11;p11), der(8)t(8;11)(p12;q13), t(11;22)(q24;q12) - presence of type-2 EWS-FLI1 hybrid transcripts (394 bp) confirmed by RT-PCR |
| Molec. Genetics ： | expression of fusion gene EWS-FLI1 confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |