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| 产品名称： | PAB-122 |
|---|---|
| 商品货号： | TS259095 |
| <strong>Cell line </strong>： | PAB-122 |
| DSMZ no. ： | TS259095 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-p53) |
| Origin ： | established by fusion of NS-1 myeloma cells and spleen cells from a male BALB/c mouse immunized with SV40-transformed, 3T3 clone 4 mouse cells; cells were reported to secrete an IgG2b antibody recognizing p53 protein present in nuclei of SV40-transformed cells |
| Reference(s) ： | 14583, 15206 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells in suspension; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS |
| Subculture ： | maintain at 0.5-5 x 105 cells/ml; split 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximal density at 1-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses ： | ELISA: reverse transcriptase negative |