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| 产品名称： | NRK-49F |
|---|---|
| 商品货号： | TS259099 |
| <strong>Cell line </strong>： | NRK-49F |
| DSMZ no. ： | TS259099 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | kidney fibroblasts |
| Origin ： | cell line was cloned from a mixed culture of normal rat kidney cells; cells exhibit contact inhibition and are very sensitive; indicator cells for viral or chemical transformation; this cell line is frequently used for TGF-ß bioassays |
| Reference(s) ： | 14626, 14452, 14362 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like cells with a fusiform shape |
| Medium ： | 95% Dulbeccos MEM + 5% h.i. FBS |
| Subculture ： | split culture 1:4 to 1:5 about 2-3 times a week using trypsin/EDTA when the cells have reached 3/4 of confluence; seed out at ca. 0.5-1.0 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of about 3.5 x 106/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | near-diploid rat karyotype with 4% polyploidy - 40(37-42)<2n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |