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| 产品名称： | 3T3 |
|---|---|
| 商品货号： | TS259103 |
| <strong>Cell line </strong>： | 3T3 |
| DSMZ no. ： | TS259103 |
| Species ： | Originally confirmed as murine by IEF, then by species-PCR and recently as Mus musculus origin by COI DNA barcoding |
| Cell type ： | fibroblasts |
| Origin ： | established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition |
| Reference(s) ： | 15016, 15015 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblasts growing adherently as monolayer with contact inhibition; image; image; imageimage; image |
| Medium ： | 90-95% Dulbeccos MEM + 5-10% h.i. FBS |
| Subculture ： | split confluent culture at a ratio of up to 1:8 every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in aggregates that are difficult to disperse; upon reaching confluence cells stop to grow; cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of about 5-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy - 68(58-73)<3n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |