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| 产品名称： | BC3H1 |
|---|---|
| 商品货号： | TS259105 |
| <strong>Cell line </strong>： | BC3H1 |
| DSMZ no. ： | TS259105 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | muscle cells from intracranial tumor |
| Origin ： | established from the offspring of a C3H-mouse injected with nitrosoethylurea during pregnancy; cells show properties characteristic of muscle cells and were described in the literature to synthesize adenylate and creatine phosphokinases, tropomyosin and myosin |
| Reference(s) ： | 14946, 16746 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, flat cells forming a confluent monolayer |
| Medium ： | 80% Dulbeccos MEM + 20 % h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every week (cells grow very slowly) using trypsin/EDTA; cell numbers may be difficult to ascertain due to formation of clumps after detachment |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | about 1-2 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | murine near-tetraploid karyotype - 74-82<4n> - 1-3 centric fusion markers and 1-2 dm present in most cells - does not correspond with published karyotype of 56 chromosomes |
| Viruses ： | ELISA: reverse transcriptase positive |