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| 产品名称： | J-774A.1 |
|---|---|
| 商品货号： | TS259107 |
| <strong>Cell line </strong>： | J-774A.1 |
| DSMZ no. ： | TS259107 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | monocytes-macrophages |
| Origin ： | established from a tumor in a female BALB/c mouse in 1968; cells were described in the literature to synthesize lysozyme and interleukin-1 and to have receptors for immunoglobulin and complement |
| Reference(s) ： | 14876 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | relatively small cells growing adherently; adherent cells will round up after a few days in culture; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 about twice a week (prior to reaching confluence); a certain amount of cells detach spontaneously if allowed to exceed confluence; adherent cells may be detached with a rubber policeman or using TEN (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl) (not trypsin/EDTA) for quantitative harvesting or for seeding out of cells collected from the supernatant for passaging; use always new culture flasks, otherwise cells are difficult to detach |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | about 4.5 x 107 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypotetraploid karyotype - 78(70-81)<4n> - in most cells a centric fragment was present - mouse chromosome morphology confirmed |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |