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| 产品名称： | PA-TU-8902 |
|---|---|
| 商品货号： | TS259108 |
| <strong>Cell line </strong>： | PA-TU-8902 |
| DSMZ no. ： | TS259108 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR fingerprinting |
| Cell type ： | pancreas adenocarcinoma |
| Origin ： | established in 1985 from the primary ductal pancreatic adenocarcinoma (grade II) from a 44-year-old woman; reported as inducing metastasis in nude mice and as secreting proteinases (e.g. urokinase, cathepsin B and D) |
| Reference(s) ： | 14509, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial adherent cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 0.5-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 25-40 hours |
| Harvest ： | cell harvest of 4-8 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded pseudotetraploid karyotype - 91(83-94)<4n>XXX-X, +5, -6, -6, +7, -10, -10, +12, -13, -13, -14, -19, +20, -22, -22, +5mar, del(4)(q26)x2, der(5)t(5;14)(p11;q11), der(5)t(5;?)(q11;?), der(7)t(7;?)(p14;?), der(9)t(9;11)(p12;q11), der(14)t(?5;14)(?p11;p11)x2, del(17)(p12), der(17)t(17;?)(p11/12;?), der(18)t(18;?)(p12;?), der(19)t(19;?)(q13.1;?), ?i(21q)x3, der(22)t(22;?)(q13;?) - carries 19p13.1 recurrent tumor breakpoint - corresponds to vague published description |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |