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| 产品名称： | HEP-G2 |
|---|---|
| 商品货号： | TS259109 |
| <strong>Cell line </strong>： | HEP-G2 |
| DSMZ no. ： | TS259109 |
| Species ： | originally confirmed as human with IEF of AST, MDH and recently by STR fingerprinting |
| Cell type ： | hepatocellular carcinoma |
| Origin ： | established from the tumor tissue of a 15-year-old Argentine boy with hepatocellular carcinoma in 1975; cell line is patented; cells were described in the literature to not harbor a hepatitis B virus genome; cells reportedly produce a variety of proteins: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement (C3, C4), C3 activator, fibrinogen, alpha1-acid glycoprotein, alpha2-HS glycoprotein, ß-lipoprotein, retinol binding protein |
| Reference(s) ： | 14673, 14347 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers and in small aggregates; image ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; after thawing, culture initially with 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/80 cm2, |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 52(47-54)<2n>XY, +2, +14, +17, +20, +2mar, t(1;21) (p22.2;p11-12), i(17q)/der(17)t(17;17)(p11;q11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |