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| 产品名称： | TN-368 |
|---|---|
| 商品货号： | TS259114 |
| <strong>Cell line </strong>： | TN-368 |
| DSMZ no. ： | TS259114 |
| Species ： | Trichoplusia ni origin confirmed by COI DNA barcoding |
| Cell type ： | larval cells |
| Origin ： | established from the larvae of a tiger moth (species Trichoplusia ni = Autographa brassicae = Plusia ni, family Noctuidae, subfamily Plusiinae, order Lepidoptera) |
| Reference(s) ： | 14609 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle-shaped cells growing in suspension (90%); cells tend to cluster in aggregates |
| Medium ： | 80% TC 100 medium + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:4 to 1:10 every 2-3; maintain at 0.4-0.5 x 106 cells/ml; after thawing, cells do not grow during the first 2-4 days and viability may drop to about 10%; seed out at ca. 0.1 x 106 cells/ml |
| Incubation ： | without exposure to CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Doubling time ： | about 24 hours |
| Harvest ： | cell harvest of about 0.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | PCR: SMRV - |