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| 产品名称： | HEPA 1-6 |
|---|---|
| 商品货号： | TS259116 |
| <strong>Cell line </strong>： | HEPA 1-6 |
| DSMZ no. ： | TS259116 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hepatoma |
| Origin ： | cell line was derived from the BW7756 tumor that arose in a C57L mouse; cells were described in the literature to secrete several liver-specific products (e.g. albumin, alpha1-antitrypsin, alpha-fetoprotein, amylase); cells may grow in serum-free medium when supplemented with dexamethasone and selenium |
| Reference(s) ： | 14445, 14446 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial cells growing as monolayer; image; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; cells grow rather quickly; seed out at ca. 1-2 x 106 cells/80 cm2 or 175 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 30 hours |
| Harvest ： | about 10-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypotetraploid karyotype with centric fusion and 6% polyploidy - flat-moded, 53-70<4n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |