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| 产品名称： | D-36 |
|---|---|
| 商品货号： | TS259119 |
| <strong>Cell line </strong>： | D-36 |
| DSMZ no. ： | TS259119 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | mast cells |
| Origin ： | established from the bone marrow of a DBA/2 mouse in 1986; cells have been described in the literature to be IL-10 sensitive |
| Reference(s) ： | 14932 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing in suspension in small clumps |
| Medium ： | 90% Iscoves MDM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1 ng/ml mouse IL-4 + 1 ng/ml mouse IL-3 |
| Subculture ： | maintain at about 0.2-8.0 x 105 cells/ml; split ratio of 1:4 every 2-3 days; viability of cells may drop to about 20% immediately after thawing but recovers after approximately 1 week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 25 hours |
| Harvest ： | maximum density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 2% tetraploidy - 40(37-41)<2n> - centric fusion only rarely present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |