微信小李
微信小章
产品简介
购买须知
| 产品名称： | IPL-LD-65Y |
|---|---|
| 商品货号： | TS259121 |
| <strong>Cell line </strong>： | IPL-LD-65Y |
| DSMZ no. ： | TS259121 |
| Species ： | confirmed as not murine and not primate with IEF of AST, MDH, PEP B |
| Cell type ： | larval cells |
| Origin ： | established from the larvae of a gypsy moth (species Lymantria dispar, family Lymantriidae, order Lepidoptera) (Schwammspinner) |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large cells; up to 30% grow adherent with processes; suspension cells are round to oval; image; image |
| Medium ： | 80% TC 100 medium + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture and grow slowly! split confluent culture 1:4 every 4-5 days using a (plastic) cell scraper |
| Incubation ： | without exposure to CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Harvest ： | cell harvest of up to 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |