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| 产品名称： | COLO-680N |
|---|---|
| 商品货号： | TS259122 |
| <strong>Cell line </strong>： | COLO-680N |
| DSMZ no. ： | TS259122 |
| Species ： | originally confirmed as human with IEF of AST, MDH and recently by STR typing |
| Cell type ： | esophagus squamous cell carcinoma |
| Origin ： | derived from the esophagus squamous cell carcinoma of a 57-year-old black woman in 1985 via passage through a nude mouse |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:2 to 1:3 every 2-3 days using trypsin/EDTA; |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60-70 hours |
| Harvest ： | cell harvest of about 10-12 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hyperdiploid/hypotriploid karyotype with 13.5% polyploidy - 53-58<2n>X, -X, +1, +1, -3, -5, -5, +6, +7, +12, -13, -14, -14, -16, -16, -18, -20, -21, -22, +16mar - add(1)(p35)x1-2, add(1)(q11)x1-2, add(1)(p11), add(3)(p11), add(4)(q35), add(7)(q35)x1-2, add(7)(p22)del(7)(q11q21), der(9)t(5;9)(q14;p11), del(11)(p11), add(12)(q24), add(19)(p13), add(19)(q13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |