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| 产品名称： | SPC-BM-36 |
|---|---|
| 商品货号： | TS259126 |
| <strong>Cell line </strong>： | SPC-BM-36 |
| DSMZ no. ： | TS259126 |
| Species ： | Spodoptera frugiperda origin determined by COI DNA barcoding |
| Cell type ： | larval cells |
| Origin ： | derived from larvae of a silkworm (species Bombyx mori, family Bombycidae, order Lepidoptera); however, COI DNA barcoding revealed SPC-BM-36 as originating from a fall armyworm (Spodoptera frugiperda) and not from a silkworm as described |
| Reference(s) ： | 14, 17 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes |
| Medium ： | 80% TC 100 medium + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper |
| Incubation ： | cells grow without CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Doubling time ： | ca. 50-100 hours |
| Harvest ： | cell harvest of up to 12 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |