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| 产品名称： | XTH-2 |
|---|---|
| 商品货号： | TS259128 |
| <strong>Cell line </strong>： | XTH-2 |
| DSMZ no. ： | TS259128 |
| Species ： | confirmed as Xenopus spec. by a species-PCR and as Xenopus laevis origin by COI DNA Barcoding |
| Cell type ： | heart endothelium cells |
| Origin ： | established from the embryonal (stage 52/53) normal heart endothelium (tadpole heart) of a South African clawed frog (Xenopus laevis Daudin) in 1979; cells were described in the literature to synthesize factor-VIII-related antigen (AHG-a.p.), fibronectin and laminin and to be tetraploid (72 +/-5, 2N = 36) |
| Reference(s) ： | 14935, 14933, 14934 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large epitheloid (low density monolayer), cobblestone with occasional piling-up (high density monolayer) |
| Medium ： | 90% Dulbeccos MEM (diluted with 20% distilled water) + 10% h.i. FBS + 20-30 mM HEPES |
| Subculture ： | split confluent culture 1:2 every 10-12 days using trypsin/EDTA; avoid centrifugation, just dilute the harvested cells with medium and seed into a new flask; cells grow extremely slowly with a doubling time of about 10-12 days; thaw in cold water (10 °C), dilute with 10-fold volume of fresh culture medium, centrifuge gently (about 80 x g for 5 min) |
| Incubation ： | keep at room temperature in closed flasks (without exposure to CO2) |
| Doubling time ： | ca. 10-12 days |
| Harvest ： | cell harvest of about 2.5 x 106 cells/80 cm2 |
| Storage ： | frozen with 90% complete medium + 10% glycerol (not DMSO) |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses ： | PCR: SMRV - |