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| 产品名称： | PAB-100 |
|---|---|
| 商品货号： | TS259130 |
| <strong>Cell line </strong>： | PAB-100 |
| DSMZ no. ： | TS259130 |
| Species ： | originally confirmed as mouse with IEF of AST, NP, PEP B and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-SV40 large T viral antigen) |
| Origin ： | established by fusion of NS-1 myeloma cells and spleen cells from a female BALB/c mouse immunized with SVT-2, an SV40-transformed BALB/c mouse cell line; cells reportedly secrete an IgG1 antibody recognizing denaturation-sensitive antigenic determinants unique to large T antigen |
| Reference(s) ： | 14455, 14583 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells growing in suspension |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS + non-essential amino acids |
| Subculture ： | optimal split ratio 1:2 every 2-3 days; maximum density of about 1.0 x 106 cells/ml should not be exceeded |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | cell harvest of about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | predominantly mouse chromosomal morphology with 3 iso-chromosome markers present in most cells - 140(134-155) |
| Viruses ： | ELISA: reverse transcriptase positive |