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| 产品名称： | LN-405 |
|---|---|
| 商品货号： | TS259131 |
| <strong>Cell line </strong>： | LN-405 |
| DSMZ no. ： | TS259131 |
| Species ： | confirmed as human with IEF of AST, G6PD, MDH |
| Cell type ： | astrocytoma |
| Origin ： | glioblastoma cells established from an astrocytoma tumor (grade IV) of a 62-year-old woman in 1986; cells were described as expressing mRNA for both TGF-ß1 and G-TSF/TGF-ß2 (but only the latter polypeptide was secreted) and expressing GFAP protein |
| Reference(s) ： | 14395 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastic, grows as monolayers in culture |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 every 4-6 days using trypsin/EDTA; cells grow rather slowly; seed out at ca. 1.5 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of about 3-4 x 106 cells/175 cm2 or 1.5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype with 37% polyploidy and with 1-3 supernumerary dm present in most cells - 102-108<4n>XXXX, +1, +2, +3, +4, +5, +7, -10, +11, +13, -16, -16, -16, +19, +20, +20, +21, +22, +8mar, del(1)(q41)x2, der(1)t(1;3)(p32;p11), add(4)(p11), add(7)(p14), add(10)(p13), i(13q) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |