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| 产品名称： | CAL-39 |
|---|---|
| 商品货号： | TS259135 |
| <strong>Cell line </strong>： | CAL-39 |
| DSMZ no. ： | TS259135 |
| Species ： | originally confirmed as human with IEF of AST, MDH, later by DNA fingerprinting |
| Cell type ： | vulva squamous cell carcinoma |
| Origin ： | established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice |
| Reference(s) ： | 14565 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayer; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 0.5 nM hydrocortisone + 1 µg/100 ml EGF |
| Subculture ： | cells are difficult to culture! Seed out 0.8-1 x 106 /80 cm2; split confluent culture 1:3 to 1:4 every 5-10 days using trypsin/EDTA (cells may need 10 min or even longer exposure to trypsin) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 50-70 hours |
| Harvest ： | cell harvest of about 4-6 x 106cells /175cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 27% polyploidy - 59(54-60)<3n>XX, -X, +1, +1, +2, +3, +9, +12, +12, +16, +20, +22, +4mar, del(1)(q22), del(3)(p14), add(4)(p16), del(7)(q32), add(9)(p22), del(12)(q15), add(12)(q24), add(18)(q23) - additional 11q rearrangements present in markers - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |