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| 产品名称： | LF-CL2A |
|---|---|
| 商品货号： | TS259140 |
| <strong>Cell line </strong>： | LF-CL2A |
| DSMZ no. ： | TS259140 |
| Species ： | confirmed as rat by IEF and species PCR, and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatocarcinoma |
| Origin ： | a primary liver carcinoma was induced by dimethyl-aminoazobenzene (DAB) in a Wistar-Commentry rat; the hepatoma was transferred to a syngeneic rat and a cell line was established and subcloned in 1966 |
| Reference(s) ： | 15147, 14422, 14601 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayers |
| Medium ： | 90-95% Dulbeccos MEM + 5-10% h.i. FBS |
| Subculture ： | split confluent culture about every 2 days using trypsin/EDTA; cells grow quickly; seed about 1 x 106 cells into a 80 cm2 flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-25 hours |
| Harvest ： | cell harvest of about 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | rat flat-moded near-triploid karyotype - 58-67<3n> - large submetacentric marker present in most cells |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |