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| 产品名称： | EBL |
|---|---|
| 商品货号： | TS259143 |
| <strong>Cell line </strong>： | EBL |
| DSMZ no. ： | TS259143 |
| Species ： | originally confirmed as bovine with IEF of G6PD, NP and recently as Bos taurus by COI DNA Barcoding |
| Cell type ： | embryonic lung cells |
| Origin ： | established from lung of a 7-month-old bovine fetus; useful for detection of Pasteurella multocida toxine |
| Reference(s) ： | 14373, 14907, 15189 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | rectangular-like adherent cells growing as monolayers |
| Medium ： | 85% RPMI 1640 or MEM (with Earles salts) + 15% h.i. FBS |
| Subculture ： | split confluent culture every 3-4 days using trypsin/EDTA; for the test cells should be starved and split once a week; seed out at about 1 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | apparently bovine karyotype with bimodal ploidy distribution - 42(40-42)<2n>/80(77-84)<4n> - structurally rearranged with apparent reduction of ch number by centric fusion followed by tetraploidization |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |