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| 产品名称： | EFO-27 |
|---|---|
| 商品货号： | TS259146 |
| <strong>Cell line </strong>： | EFO-27 |
| DSMZ no. ： | TS259146 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR fingerprinting |
| Cell type ： | ovary adenocarcinoma |
| Origin ： | established from the solid omental metastasis of a mucinous papillary adenocarcinoma of the ovary of a 36-year-old woman in 1979; initially diploid, later tetraploid; growth in nude mice |
| Reference(s) ： | 14697, 14961 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 0.5-1.5 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 every 2-5 days using trypsin/EDTA (incubate for 10-20 minutes with trypsin/EDTA); |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | cell harvest of about 0.3-0.6 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 17% polyploidy - 78-91<4n>XXXX, -4, -10, -13, -13, -19, +mar - i(9q), 16qh+x2 - extensive subclonal rearrangements, e.g. der(5)t(5;7)(q31.2;q11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |