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| 产品名称： | COLO-824 |
|---|---|
| 商品货号： | TS259151 |
| <strong>Cell line </strong>： | COLO-824 |
| DSMZ no. ： | TS259151 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural fluid of a 52-year-old woman with breast carcinoma in 1991 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow loosely adherent in monolayers, some cells grow as round cells in clumps in suspension; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | split 1:2 to 1:3 every 2-4 days without use of trypsin/EDTA (cells are only loosely adherent); seed out at ca. 3-5 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-80 hours |
| Harvest ： | cell harvest of about 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12)t(12;?)(p13;?), der(15)t(15;?)(p12;?), der(17)t(17;?)(p12;?) - carries small bisatellited marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |