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| 产品名称： | COLO-704 |
|---|---|
| 商品货号： | TS259152 |
| <strong>Cell line </strong>： | COLO-704 |
| DSMZ no. ： | TS259152 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | ovarian adenocarcinoma |
| Origin ： | established from the ascites fluid stemming from the colon metastasis of a 46-year-old white woman with ovarian adenocarcinoma in 1986 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | grows as clusters and single cells in suspension |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-3 days; seed out at ca. 0.2-0.3 x 106 cells/ml |
| Incubation ： | 5% CO2 (or may be grown in closed flasks), 37 °C |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | saturation density at about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 22% polyploidy - 51(48-53)<2n>XX, +1, +7, +8, +8, -17, +19, +20, i(1q), der(8)t(8;?)(q21;?) - dm present in 16% cells - i(1q) is associated with ovarian carcinoma - identification confirmed |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |