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| 产品名称： | NRK-52E |
|---|---|
| 商品货号： | TS259153 |
| <strong>Cell line </strong>： | NRK-52E |
| DSMZ no. ： | TS259153 |
| Species ： | confirmed as rat with IEF of AST, MDH, NP, PEP B and by species PCR |
| Cell type ： | kidney epithelial-like cells |
| Origin ： | cell line was cloned from a mixed culture of normal rat kidney cells as was NRK-49F (DSM ACC 172); the two cell lines were described in the literature to be distinct in growth properties and in transforming abilities; NRK-52E is supposed to be much more stable |
| Reference(s) ： | 14452 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers |
| Medium ： | 90-95% Dulbeccos MEM + 5-10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | cell harvest of about 3-5 x 106/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | near-diploid rat karyotype with 4% tetraploidy - 41/42(36-42)<2n>XX - hypodiploid sideline with 1q+ marker (18%) - near triploid sideline with additional structural and numerical rearrangements (2%) - presence of sidelines-subclones may warrant cloning prior to use in transformation assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |