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| 产品名称： | L-428 |
|---|---|
| 商品货号： | TS259155 |
| <strong>Cell line </strong>： | L-428 |
| DSMZ no. ： | TS259155 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established from the pleural effusion of a 37-year-old woman with Hodgkin lymphoma (stage IVB, nodular sclerosis, refractory, terminal) in 1978. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14924, 14478, 14923, 15213, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells in suspension or as clusters; polymorph cells, some cells have "spikes", 1-5% giant multinucleated cells; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; after an initial slow start cells grow quickly; maintain at 0.2-0.5 x 106 cells/ml; split culture 1:2 every 2-3 days; viability may vary between 70% and 90% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | saturation density at about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD25 -, CD30 +, CD34 -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 10% polyploidy - 94(75-99)<4n>XXXX, +2, -5, +6, -9, -9, +12, -13, +17, +4mar, +2r/dmin, del(1)(q13), der(2)t(2;4)(p14;q21)x2-3, add(5)(p11), add(6)(q24/25)x2, add(7)(q35)x2, del(7)(p14), add(9)(p22), del(11)(q22.3), del(12)(q21)x1-2, add(13)(p11)x2, add(14)(q32)x2, der(16)t(16;?)(q24;?)t(1;?)(q11;?), del(17)(p12), del(21)(q21.2)x2 - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene ELMO1-SLCO3A1 confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |