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| 产品名称： | COLO-699 |
|---|---|
| 商品货号： | TS259156 |
| <strong>Cell line </strong>： | COLO-699 |
| DSMZ no. ： | TS259156 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | lung adenocarcinoma |
| Origin ： | established from the pleural fluid of a 57-year-old woman with adenocarcinoma of the lung in 1986 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid cells growing as monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out about 1-2 x 106 cells/175 cm2; split confluent cultures every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | cell harvest of about 12 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 2% polyploidy - 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(16q), der(21)t(21;?)(p12;?)x2 - a supernumerary small ring chromosome was present in 28% cells - corresponds to original karyotype with reinterpretation |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |