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| 产品名称： | SK-N-MC |
|---|---|
| 商品货号： | TS259161 |
| <strong>Cell line </strong>： | SK-N-MC |
| DSMZ no. ： | TS259161 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | neuroblastoma |
| Origin ： | established from the supraorbital metastasis of a neuroblastoma of a 14-year-old girl in 1971 (see ATCC HTB 10); although described in the original paper as neuroblastoma, SK-N-MC is now widely regarded as having originated from the morphologically similar Askins tumor related to Ewings sarcoma; FLI1-EWS gene fusion, the molecular pathological change in Ewings sarcoma and Askins tumor, was shown in SK-N-MC by Dunn et al. |
| Reference(s) ： | 14976, 14388, 15225 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblast-like cells growing as monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 1-4 x 106 cells/80 cm2; cells may grow slowly initially |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of about 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 6% polyploidy - 46(40-47)<2n>XX, +8, -16, t(1;4)(p36;p13), t(2;3)(q24;q29), der(3)t(3;3)(p13;q22), del(10)(q25), t(11;22)(q24;q12), t(15;22)(q21;q12), add(17)(p12), add(21)(p12) - dmin in 9% of cells - resembles published karyotype - carries masked 11;22 translocation recurrent in Ewings and Askins tumor |
| Molec. Genetics ： | expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |