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| 产品名称： | PA-TU-8988S |
|---|---|
| 商品货号： | TS259162 |
| <strong>Cell line </strong>： | PA-TU-8988S |
| DSMZ no. ： | TS259162 |
| Species ： | originally confirmed as human with IEF of AST, MDH and later by STR typing |
| Cell type ： | pancreas adenocarcinoma |
| Origin ： | established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman (sister cell line is PA-TU-8988T, ACC-no.: 162) |
| Reference(s) ： | 14508 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers |
| Medium ： | 90% Dulbeccos MEM + 5% h.i. FBS + 5% horse serum + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2-4 x 106 cells/80 cm2; split confluent culture ca. 1:4 - 1:8 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 50-60 hours |
| Harvest ： | cell harvest of about 30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as PA-TU-8988Tusing multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near triploid karyotype with 6% polyploidy - 71(67-77)<3n>XXX, +3, -4, +7, -8, +9, +11, +11, -15, -17, -18, -18, -19, -21, -22, +6mar, del(1)(p21), del(3)(p11p22), del(6)(q26), del(9)(p12), dup(11)(p14->pter), del(12)(p13), add (15)(p12) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |