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| 产品名称： | 2M6 |
|---|---|
| 商品货号： | TS259164 |
| <strong>Cell line </strong>： | 2M6 |
| DSMZ no. ： | TS259164 |
| Species ： | confirmed as mouse with IEF of LDH, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-chicken retina) |
| Origin ： | cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with Triton X114-purified chicken retina E10 proteins; described as secreting a mouse monoclonal antibody (IgG) reacting with chicken retina (Mueller glia cell-specific antigen) |
| Reference(s) ： | 14939, 14940 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clumps in suspension, some cells are slightly adherent |
| Medium ： | 80-85% RPMI 1640 + 10-15% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing |
| Subculture ： | cells are difficult to culture! maintain at ca. 0.2-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml; after thawing cultivate cells for the first 1-2 weeks in 24- or 12-well-plates using HFCS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | maximal density at ca. 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of IgG1 monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |