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| 产品名称： | 3T6 |
|---|---|
| 商品货号： | TS259165 |
| <strong>Cell line </strong>： | 3T6 |
| DSMZ no. ： | TS259165 |
| Species ： | originally confirmed as mouse with IEF of AST, G6PD, MDH, NP, by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | fibroblasts |
| Origin ： | established from disaggregated Swiss albino mouse embryos in 1963; cells were described in the literature to secrete collagen and hyaluronic acid |
| Reference(s) ： | 15015, 15016 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblasts growing adherently as monolayer |
| Medium ： | 85-90% Dulbeccos MEM + 10-15% h.i. FBS |
| Subculture ： | split confluent culture ca. 1:10 every 2-3 days using trypsin/EDTA, seed out about 2 x 106 cells/175 cm2; cells may clump after trypsinization and may be difficult to disperse |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 15 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine flat-moded hypertriploid/hypotetraploid karyotype - 67(64-73)<3n> |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |