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| 产品名称： | NB-4 |
|---|---|
| 商品货号： | TS259172 |
| <strong>Cell line </strong>： | NB-4 |
| DSMZ no. ： | TS259172 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | acute promyelocytic leukemia |
| Origin ： | established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; patented cell line; cells carry the t(15;17) PML-RARA fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14480, 14496, 14705, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells in suspension, round and polymorphic cells, very few are giant cells; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio at 1:2 to 1:3 every 2-3 days; viability may be low after thawing, but cells will recover soon when cultured initially with 20% FBS; cells might also grow better in 24-well-plates than in culture flasks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-45 hours |
| Harvest ： | cell harvest of 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD11b -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD38 +, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 3% polyploidy - 78(71-81)<3n>XX, -X, +2, +6, +7, +7, +11, +12, +13, +14, +17, -19, +20, +4mar, der(8)t(8;?)(q24;?), der(11)t(11;?)(?->::11p15->11q22.1::11q13->22.1:), der(12)t(12;?)(p11;?), 14p+, t(15;17)(q22;q11-12.1), der(19)t(10;19)(q21.1;p13.3)x2 - identity confirmed |
| Molec. Genetics ： | expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |