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| 产品名称： | MC3T3-E1 |
|---|---|
| 商品货号： | TS259174 |
| <strong>Cell line </strong>： | MC3T3-E1 |
| DSMZ no. ： | TS259174 |
| Species ： | originally confirmed as mouse with IEF of AST, MDH, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | embryo/fetus calvaria fibroblasts |
| Origin ： | established from the calvaria of an embryo/fetus C57BL/6 mouse; described in the literature to differentiate to osteoblasts and to produce collagen |
| Reference(s) ： | 14674 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblast-like cells growing as monolayer; image; image |
| Medium ： | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at about 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 1-2 days |
| Harvest ： | cell harvest of ca. 6-7 x 106 cells/80 cm2 or 9 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypotetraploid karyotype with 11.5% polyploidy - 72(68-80)<4n> - centric fusion absent |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |