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| 产品名称： | SH-SY5Y |
|---|---|
| 商品货号： | TS259175 |
| <strong>Cell line </strong>： | SH-SY5Y |
| DSMZ no. ： | TS259175 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR-fingerprinting |
| Cell type ： | neuroblastoma |
| Origin ： | clonal subline of the neuroepithelioma cell line SK-N-SH that had been established in 1970 from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma |
| Reference(s) ： | 14388, 14638, 14901 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-/neuronal-like elongated cells growing as monolayer and in cell clusters; cells do not grow to complete confluency; image |
| Medium ： | 80-85% Dulbeccos MEM + 15-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split culture 1-2 times a week using trypsin/EDTA; selection of neuronal-like cells may be favored by reduction of glutamine (1 mM), passaging of semi- or half-confluent cultures; short-time trypsin exposure preferentially detaches the epithelial-like cells |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 1.8% polyploidy - 46/47(42-48)<2n>X/XX, +7, ins(1)(q32q11q43), add(9)(q34), der(22)t(?17;22)(q22;q13) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |