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| 产品名称： | BF-F3 |
|---|---|
| 商品货号： | TS259179 |
| <strong>Cell line </strong>： | BF-F3 |
| DSMZ no. ： | TS259179 |
| Species ： | originally confirmed as mouse with IEF of AST, NP, PEP B and species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | this hybridoma cell line was described as secreting a mouse monoclonal antibody (IgM) reacting with adult rat type 2B fibers in immunofluorescence and with rat type 2B MHCs (myosin heavy chain) in Western blotting; established in 1983 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles |
| Reference(s) ： | 14929 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells in suspension and partially adherent, but trypsin/EDTA is not necessary to detach the cells |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | maintain at 0.2-0.5 x 106 cells/ml, optimal split ratio of 1:2 to 1:4 every 2-4 days; seed out at ca. 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | 20-30 hours |
| Harvest ： | maximum density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine chromosome morphology |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |