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| 产品名称： | B9 |
|---|---|
| 商品货号： | TS259184 |
| <strong>Cell line </strong>： | B9 |
| DSMZ no. ： | TS259184 |
| Species ： | originally confirmed as mouse with IEF of AST, PEP B and by species PCR and as mus musculus origin by COI DNA barcoding |
| Cell type ： | hybridoma |
| Origin ： | cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer; cells are IL-6-dependent |
| Reference(s) ： | 14342 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS + 50 µM mercaptoethanol + 50-100 pg/ml human IL-6 |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml, best in 24-well plate; add IL-6 every 1-2 days in the first week; cells have a high metabolic activity aciditying the culture medium. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | maximal density at about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell dilutions beginning with cell densities of 2.5 x 104 cells/ml; duration of assay is commonly 2-3 days) |
| Cytogenetics ： | apparently murine chromosome morphology with extensive centric fusions |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |