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| 产品名称： | SC-71 |
|---|---|
| 商品货号： | TS259189 |
| <strong>Cell line </strong>： | SC-71 |
| DSMZ no. ： | TS259189 |
| Species ： | originally confirmed as mouse with IEF of NP, PEP B and species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) reacting with adult rat skeletal muscle type 2A (but not 2B) fibers in immunofluorescence and with undigested type 2A MHCs (myosin heavy chain) as well as both rod and light meromyosin fragments in Western blotting; rat neonatal and embryonic MHCs were not studied by Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle |
| Reference(s) ： | 14929 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | maintain at 0.1-0.5 x 106 cells/ml; split saturated culture at 1:5 to 1:10 every 2-5 days; seed out at ca. 0.5 x 106 cells/ml; use initially 24-well plate culture dishes and 20% FBS; daily exchange of medium is recommended |
| Incubation ： | at 37 °C with 7.5% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |