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| 产品名称： | LCL-WEI |
|---|---|
| 商品货号： | TS259191 |
| <strong>Cell line </strong>： | LCL-WEI |
| DSMZ no. ： | TS259191 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | B lymphoblastoid cells (from patient with melanoma) |
| Origin ： | Epstein-Barr virus-positive cell line established from peripheral blood in 1977 from male patient with melanoma |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid cells growing singly and in large clumps in suspension; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | maintain at about 0.3-1.0 x 106 cells/ml; optimal split ratio of 1:3 to 1:8 every 2-4 days; the culture usually contains a large amount of cell debris |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | saturation density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, cyIgG +, cyIgM -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype with diploid (36%) sideline and 4% polyploidy - 90(88-94)<4n>XX/XXY/XXYY - no consistent structural aberrations |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |