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| 产品名称： | COLO-849 |
|---|---|
| 商品货号： | TS259196 |
| <strong>Cell line </strong>： | COLO-849 |
| DSMZ no. ： | TS259196 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | melanoma |
| Origin ： | derived in 1992 from right axillary lymph nodes of a 43-year-old Caucasian man with metastatic maligant melanoma |
| Reference(s) ： | 14788 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastic cells growing mainly as adherent monolayer and partly in suspension; sedimented cells have a brown color (suggesting production of melanin) |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:10 every 4-7 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of about 18 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype - 60<3n>X, -Y, -1, +3, -4, -5, +7, -10, -11, -12, -13, -14, -15, -16, -17, -18, +20, +22, der(1)t(1;?)(q11;?), i(3q), idic(3)(3cen->p24::1q11->qter), der(7)t(7;?)(q33;?)x2, der(9)t(9;?)(p11;?)/del(9)(p13) - the large idic(3) marker and i(3q) derive from an earlier translocation, t(1;3)(q11;p24), present in a sideline - idic marker clearly derives from published translocation |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |