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| 产品名称： | HUP-T4 |
|---|---|
| 商品货号： | TS259199 |
| <strong>Cell line </strong>： | HUP-T4 |
| DSMZ no. ： | TS259199 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | pancreas carcinoma |
| Origin ： | established from the malignant ascites of a 60-year-old Japanese man with pancreas carcinoma in 1985 |
| Reference(s) ： | 14808 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers; image |
| Medium ： | 80% MEM (with Earles salts) + 20% h.i. FBS + 1% non-essential amino acids + 1% sodium pyruvate |
| Subculture ： | optimal split ratio of 1:2 to 1:3 every 2-5 days using trypsin/EDTA (recommended: trypsin/EDTA for 10 min at 37 °C); seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 38 hours |
| Harvest ： | cell harvest of about 1-2 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-triploid karyotype with 11% polyploidy and 1-2 supernumerary dm - 69/70(66-73)<3n>X, ?del(X)/XY, -Y, -2, -4, +6, +7, +8, -9, -9, +11, +16, +mar - inv(3)(p22q24), i(8q), idic(9)(pter), add(17)(p12) - 2 medium size ring ch present at 10% |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |