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| 产品名称： | HAP-T1 |
|---|---|
| 商品货号： | TS259204 |
| <strong>Cell line </strong>： | HAP-T1 |
| DSMZ no. ： | TS259204 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin by COI DNA barcoding |
| Cell type ： | pancreas carcinoma |
| Origin ： | established from the BHP-induced pancreatic carcinoma of a Syrian golden hamster (Mesocricetus auratus)(GN strain) in 1983 |
| Reference(s) ： | 14913 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid monolayer |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture at 1:3 every 3 days or at 1:6 every 5-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 25-30 hours |
| Harvest ： | cell harvest of about 50-60 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint ： | unique DNA profile with (gtg)5-probe, heterozygous with human single locus probe pYNH24 |
| Cytogenetics ： | near diploid hamster karyotype with tetraploid sideline (18%) and 18% polyploidy - 44(40-45)<2n>X/XX - paucity of homologous chromosomes indicates a high level of rearrangement - hamster markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |