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| 产品名称： | KASUMI-1 |
|---|---|
| 商品货号： | TS259205 |
| <strong>Cell line </strong>： | KASUMI-1 |
| DSMZ no. ： | TS259205 |
| Species ： | confirmed as human with IEF of AST, PEP B |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 7-year-old Japanese boy with acute myeloid leukemia (AML FAB M2) (in 2nd relapse after bone marrow transplantation) in 1989; cells carry the t(8;21) leading to RUNX1-RUNX1T1 (AML1-ETO) fusion gene; described in the literature to carry the KIT mutation N822. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14361, 14481, 17456, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line was shown to exhibit reverse transcriptase (RT) activity detected by RT-ELISA. However, HIV-1 and -2 or HTLV-I and -II sequences could not be detected by PCR analysis. The cell line is thus categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clumps in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | start culture with a cell concentration of >1 x 106 cells/ml and cultivate initially in 24-well plates; viability may drop below 50% within the first 24 hours after thawing; in log-phase, optimal density is at about 0.5 x 106 cells/ml and a split ratio of saturated culture about 1:2 every 2-3 days is recommended; a significant amount of cell debris is always visible in the background which might be due to granula or other intracellular material |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48-72 hours |
| Harvest ： | saturation density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, CD38 +, CD71 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype - 45<2n>X, -Y, -9, -13, -16, +3mar, t(8;21)(q22;q22), der(9)t(9;?)(p22;?), der(15)t(?9;15)((?q11;?p11) - carries both partners of 8;21 translocation associated with AML (mainly FAB M2) |
| Molec. Genetics ： | expression of fusion gene RUNX1-RUNX1T1 (AML1-ETO) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |