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| 产品名称： | NUC-5 |
|---|---|
| 商品货号： | TS259208 |
| <strong>Cell line </strong>： | NUC-5 |
| DSMZ no. ： | TS259208 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | urinary bladder |
| Origin ： | established from an adult female C3H/Law mouse; non-tumorigenic in vitro immortalized urine bladder cell line with aberrant growth factor response, aneuploid (chromosome number 70-75) |
| Reference(s) ： | 14449 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large and flat regular arranged cells, growing in up to 3 cell layers |
| Medium ： | 45% Hams F10 + 45% Dulbeccos MEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 µM HEPES |
| Subculture ： | split confluent culture 1:4 every 6-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60-75 hours |
| Harvest ： | harvest of 2-3 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | murine hypotetraploid karyotype with 14% polyploidy and 1-3 dmin in 34% cells - 70(64-74)<4n> - 1-3 centric fusion markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |